小鼠卵巢玻璃化冷冻原位移植后子代行为学评估*

王生存1,2**,孙 3,李碧春1,邵义祥2,朱顺星2,王庆华2,高 2,郭 2,3***

(1扬州大学动物科学与技术学院,扬州 2250092南通大学实验动物中心;3南通大学附属医院生殖医学中心)

[ ] 目的:建立小鼠卵巢玻璃化冷冻原位移植模型,通过对后代的行为学检测,探讨卵巢玻璃化冷冻的长期安全性。方法:选择8周龄的C57BL/6小鼠,将卵巢取出进行玻璃化冷冻,8周后复苏,同种异体原位移植,接受冷冻卵巢移植的母鼠通过自然交配所生后代作为实验对象进行行为学检测,以正常C57BL/6小鼠为对照。结果:6项经典行为学测试结果显示,卵巢玻璃化冷冻复苏移植后出生的子代小鼠的行为学表现与对照组比较差异无统计学意义(P0.05)。结论:卵巢玻璃化冷冻复苏原位移植可使受体母鼠恢复自然生育能力,其后代在行为学方面未见不良影响,是一种安全的生殖力保存方法。

[关键词] 卵巢玻璃化冷冻;生育力保存;卵巢原位移植;行为学检测;C57BL/6小鼠

[中图分类号] R339.2 [文献标志码] A [文章编号] 1674-7887201803-0161-05

The behavioral assessment of the mice born from the orthotopic transplantation of vitrified ovaries*

WANG Shengcun1,2**, SUN Hua3, LI Bichun1, SHAO Yixiang2, ZHU Shunxing2, WANG Qinghua2, GAO Yuan2, GUO Feng2,3***(1College of Animal Science and Technology, Yangzhou University, Yangzhou 225009; 2Laboratory Animal Center, Nantong University; 3Center for Reproductive Medicine, the Affiliated Hospital of Nantong University)

[Abstract] Objective: A mouse model of vitrified ovaries orthotopic transplantation was established. The long-term safety of ovarian vitrification was explored through the offspring behavioral tests. Methods: The 8 weeks old C57BL/6 mice were selected for ovarian vitrification, 8 weeks later the ovaries were thawed and orthotopic transplanted in suit to an allogeneic female mouse. The vitrified ovary recipient female mouse was mated with C57BL/6 male, and the offsprings behavioral were tested. The normal C57BL/6 mice were used as controls. Results: 6 classic behavioral tests showed that the behavior of the offspring from vitrified ovary in situ transplantation has no significant difference compared to the control group(P0.05). Conclusion: Ovarian vitrification and in situ transplantation can restore the fertility of the recipient female mice, there have no adverse effects on the offspring behavior, it is safe for mouse behavior as a way of fertility preservation.

[Key words] ovarian vitrification; fertility preservation; ovarian in suit transplantation; behavior test; C57BL/6 mouse

* [基金项目] 江苏省妇幼保健科研项目(F201442),南通市科技计划项目(HS2013042),南通市应用基础研究科技项目(MS12015067)

** [作者简介] 王生存,女,汉族,生于19794月,江苏省南通市人,硕士,教研室副主任,研究方向:人类疾病动物模型。

*** [通信作者] 郭丰,电话:0513-81168131E-mail: guof89@yahoo.com

高促甲状腺素血症早产儿脑损伤诊断方法的临床应用*

孙雪梅1,2**,胡旻婧1,吴尤佳1,索桂海1,徐美玉1,李海英1***

(1南通大学附属医院儿科,南通 2260012江苏省泰州市人民医院儿科)

[ ] 目的:探讨振幅整合脑电图(amplitude-integrated electroencephalography, aEEG)、血清S100B蛋白、头颅磁共振成像(magnetic resonance imaging, MRI)、神经行为量表在高促甲状腺素血症(hyperthyrotropinemia, HT)早产儿脑损伤中的诊断价值。方法:将南通大学附属医院新生儿重症监护病房201312月—20156月间确诊为HT78例早产儿纳入为研究对象,出生后6 h内监测aEEG24 h检测血清S100B7~10 d检查头颅MRI,纠正胎龄满40周后71428 d评估新生儿神经行为测定(neonatal behavioral neurological assessment, NBNA)评分,纠正胎龄3612个月时检测婴幼儿智能发育量表(Childrens Developmental Center of China, CDCC),计算各指标诊断脑损伤的灵敏度、特异度,采用Kappa检验比较各指标与脑损伤的吻合度。结果:(1)aEEG的灵敏度、特异度及K值均较高,分别为91.30%94.50%84.80%(2)aEEG在生后6 h内即能有助于诊断脑损伤。结论:aEEG、血清S100B、头颅MRINBNACDCC均可用于诊断HT早产儿脑损伤,aEEG可作为超早期(6 h)有效的辅助诊断指标。

[关键词] 高促甲状腺素血症;脑损伤;振幅整合脑电图;早产儿

[中图分类号] R722.1 [文献标志码] A [文章编号] 1674-7887201803-0166-04

Clinical application of diagnostic methods for brain damage in premature infants with hyperthyroidism*

SUN Xuemei1,2**, HU Minjing1, WU Youjia1, SUO Guihai1, XU Meiyu1, LI Haiying1*** (1Department of Pediatrics, the Affiliated Hospital of Nantong University, Nantong 226001; 2Department of Pediatrics, Jiangsu Taizhou Peoples Hospital)

[Abstract] Objective: To discuss the diagnostic value of amplitude-integrated electroencephalography(aEEG), serum S100B protein, magnetic resonance imaging(MRI), and nerve behavior scale in the brain injury of preterm infants with hyperthyrotropinaemia(HT). Methods: The clinical data of 78 cases of premature infants diagnosed with HT from December 2013 to June 2015 were enrolled in the study, 6 hours aEEG and 24 h serum S100B protein after birth. Head MRI examination at 7-10 days, neonatal behavioral neurological assessment(NBNA) respectively at the 7, 14 and 28 days after corrected gestational age over 40 weeks and correcting gestational age 3, 6, and 12 months for Childrens Developmental Center of China(CDCC). Calculating sensitivity and specificity of each indicator in diagnosing brain injury, Comparing the indexes of each index and the degree of brain injury by Kappa test. Results: (1)The sensitivity, specificity and K values of aEEG were higher, 94.50%, 91.30% and 84.80% respectively. (2)aEEG can help diagnose brain damage within 6 hours after birth. Conclusion: AEEG, serum S100B, head MRI, NBNA and CDCC can be used for the diagnosis of brain damage in premature infants with hyperthyroidism, and aEEG can be used as an auxiliary diagnostic marker for super early(6 hours).

[Key words] hyperthyrotropinaemia; brain injury; amplitude-integrated electroencephalography; premature infant

* [基金项目] 江苏省妇幼健康科研项目(F201405, F201649),南通市卫计委科技项目(WQ2016071),南通市科技计划项目(MS12015002)

** [作者简介] 孙雪梅,女,汉族,生于198911月,江苏省泰州市人,硕士,研究方向:早产儿发育脑损伤。

*** [通信作者] 李海英,电话:13912266605E-mail: zl070619@126.com